Membrane:Article Title: Hsa_circ_0058495-mediated IGF2BP2 ubiquitination and m6A modification of MEKK1 promote the progression of PDAC
Article Snippet: .. Subsequently, the protein was deposited onto a PVDF membrane and incubated overnight with the following primary antibodies at 4 °C: anti-IGF2BP2 antibody (14672, CST), anti-EIF4A3 antibody (bs-14548R, Bioss), MAPK Family antibody sampler kit (9926, CST), phospho-MAPK family antibody sampler kit (9910, CST), anti-ubiquitin antibody (ab134953, Abcam), anti-K48 antibody (4289, CST), anti-Flag antibody (66008-4-Ig, Proteintech), anti-HA antibody (51064-2-AP, Proteintech), anti-LC3A/B antibody (12741, CST), anti-SQSTM1 antibody (sc-26675, Santa Cruz), anti-TRIM25 antibody (67314-1-Ig, Proteintech), anti-Arginase-1 antibody (16001-1-AP, Proteintech), anti-iNOS antibody (18985-1-AP, Proteintech), anti-CD206 antibody (81525-1-RR, Proteintech), anti-CD86 antibody (13395-1-AP, Proteintech), anti-α-Tubulin antibody (sc-8035, Santa Cruz), anti-β-actin antibody (1030300012, lifespaceo). ..
Incubation:Article Title: Hsa_circ_0058495-mediated IGF2BP2 ubiquitination and m6A modification of MEKK1 promote the progression of PDAC
Article Snippet: .. Subsequently, the protein was deposited onto a PVDF membrane and incubated overnight with the following primary antibodies at 4 °C: anti-IGF2BP2 antibody (14672, CST), anti-EIF4A3 antibody (bs-14548R, Bioss), MAPK Family antibody sampler kit (9926, CST), phospho-MAPK family antibody sampler kit (9910, CST), anti-ubiquitin antibody (ab134953, Abcam), anti-K48 antibody (4289, CST), anti-Flag antibody (66008-4-Ig, Proteintech), anti-HA antibody (51064-2-AP, Proteintech), anti-LC3A/B antibody (12741, CST), anti-SQSTM1 antibody (sc-26675, Santa Cruz), anti-TRIM25 antibody (67314-1-Ig, Proteintech), anti-Arginase-1 antibody (16001-1-AP, Proteintech), anti-iNOS antibody (18985-1-AP, Proteintech), anti-CD206 antibody (81525-1-RR, Proteintech), anti-CD86 antibody (13395-1-AP, Proteintech), anti-α-Tubulin antibody (sc-8035, Santa Cruz), anti-β-actin antibody (1030300012, lifespaceo). ..
Ubiquitin Proteomics:Article Title: Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DEAD-box helicase 5–activating transcription factor 1–protein phosphatase 2 regulatory subunit 5A signaling
Article Snippet: .. NF-κB Pathway Sampler Kit (9936, Cell Signaling Technology), phospho-MAPK Family Antibody Sampler Kit (9910, Cell Signaling Technology), MAPK Family Antibody Sampler Kit (9926, Cell Signaling Technology), TLE3 (11372–1-AP, Proteintech), DDX5 (10804–1-AP, Proteintech), α-tubulin (11224–1-AP, Proteintech), HA-tag (51064–2-AP, Proteintech), Myc-tag (60003–2-Ig, Proteintech), PPP2R5A (12675–2-AP, Proteintech), ZF5 (22056–1-AP, Proteintech), E2F1 (66515–1-Ig, Proteintech), E2F4 (10923–1-AP, Proteintech), E2F7 (24489–1-AP, Proteintech), ATF1 (11946–1-AP, Proteintech), CREB1 (12208–1-AP, Proteintech), FOXO4 (AF6369, Affinity), Flag-tag (66008–4-Ig, Proteintech), PPP1CB (55136–1-AP, Proteintech), PPP3CA (13422–1-AP, Proteintech), PPP5C (11715–1-AP, Proteintech), TAK1 (12330–2-AP, Proteintech), p-TAK1 (AP0071, ABclonal), Pan Acetyl-Lysine (A1525, ABclonal), Ubiquitin (20326, Cell Signaling Technology), IRF3 (11312–1-AP, Proteintech), NLRP3 (19771–1-AP, Proteintech), JUN (24909–1-AP, Proteintech), phospho-IRF-3 (Ser379) (79945, Cell Signaling Technology), Cleaved Caspase-1 (Asp296) (89332, Cell Signaling Technology), Cleaved-IL-1β (Asp116) (83186, Cell Signaling Technology), Cleaved Gasdermin D (Asp275) (36425, Cell Signaling Technology), Caspase11 p20 (sc-374615, Santa Cruz Biotechnology). .. The secondary antibodies used were Anti-rabbit IgG and Anti-mouse IgG, both from Cell Signaling Technology.
Article Title: Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DEAD-box helicase 5-activating transcription factor 1-protein phosphatase 2 regulatory subunit 5A signaling.
Article Snippet: .. NF-jB Pathway Sampler Kit (9936, Cell Signaling Technology), phospho-MAPK Family Antibody Sampler Kit (9910, Cell Signaling Technology), MAPK Family Antibody Sampler Kit (9926, Cell Signaling Technology), TLE3 (11372–1-AP, Proteintech), DDX5 (10804–1-AP, Proteintech), atubulin (11224–1-AP, Proteintech), HA-tag (51064–2-AP, Proteintech), Myc-tag (60003–2-Ig, Proteintech), PPP2R5A (12675–2-AP, Proteintech), ZF5 (22056–1-AP, Proteintech), E2F1 (66515–1-Ig, Proteintech), E2F4 (10923–1-AP, Proteintech), E2F7 (24489–1-AP, Proteintech), ATF1 (11946–1-AP, Proteintech), CREB1 (12208–1- AP, Proteintech), FOXO4 (AF6369, Affinity), Flag-tag (66008–4-Ig, Proteintech), PPP1CB (55136–1-AP, Proteintech), PPP3CA (13422– 1-AP, Proteintech), PPP5C (11715–1-AP, Proteintech), TAK1 (12330–2-AP, Proteintech), p-TAK1 (AP0071, ABclonal), Pan Acetyl-Lysine (A1525, ABclonal), Ubiquitin (20326, Cell Signaling Technology), IRF3 (11312–1-AP, Proteintech), NLRP3 (19771–1- AP, Proteintech), JUN (24909–1-AP, Proteintech), phospho-IRF-3 (Ser379) (79945, Cell Signaling Technology), Cleaved Caspase-1 (Asp296) (89332, Cell Signaling Technology), Cleaved-IL-1b (Asp116) (83186, Cell Signaling Technology), Cleaved Gasdermin D (Asp275) (36425, Cell Signaling Technology), Caspase11 p20 (sc-374615, Santa Cruz Biotechnology). .. The secondary antibodies used were Anti-rabbit IgG and Anti-mouse IgG, both from Cell Signaling Technology.
Western Blot:
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